P-89: Role of Nutrition Diet with and without Mineral-Vitamin Elements on Superovulation of Mice

Background: The quality and quantity of the diet has undeniable effects on the reproduction of organisms. We examined the effects of two diets (with and without mineral supplementation-Vitamins) on super-ovulation in two strains of mice (B6D2F1 and NMRI) in Royan Institute, Tehran. Materials and Methods: For this purpose, 40 female mice (21 days) from each strain were divided into four experimental groups (n =10) including: (1) NMRI mice with diet supplements, (2) in NMRI without supplements (3) B6D2F1 mice with diet supplements and (4) B6D2F1 without supplements. The animal received food and water ad libitum in controlled standard conditions. At the age of six weeks, mice were treated (IP) with 7/5 IU PMSG and 7/5 IU hCG. The next morning, after cervical dislocation, all of the oocytes were counted. Data collected for each group included the total numbers of oocytes and the numbers of dead oocytes. The data were analyzed using the software SPSS16. Results: At the beginning of the period (21 days), there was no significant difference in weight between different strain (Average Weight 10/8 g). Weight differences between the strains that started from age 42 days to 49 days continued (end of period) (P <0/05). NMRI (mean weight at the end of period 25 g) compared with B6D2F1 (mean weight end of period 18/5 mg), despite identical initial weight and eating, at 49 days of age were significantly more weight. On the other hand, the rate of ovulation between experimental groups were similar, but the rate of degeneration of oocyte in NMRI with diet supplements (mean = 2/5) were significantly less than NMRI without supplements (mean = 6/1) and B6D2F1 with diet supplements (mean = 6). Also, rate of degeneration in B6D2F1 without supplements compared with other groups (3/5) were no difference. Conclusion: Result of this study demonstrates that significantly decreased degeneration of oocyte in NMRI mice than in B6D2F1 can be interpreted on the basis of genetic differences and differences in nutritional requirements.